Download Category: BIO-CHEMISTRY PROJECT TOPICS
ISOLATION AND CHARACTERIZATION OF MICROORGANISMS FROM STORED PAP (OGI)
ABSTRACT The microbial changes which took place during the steeping and storage of pan (ogi) was studied. The steeped water had an initial PH of…
GROWTH PERFORMANCE AND BLOOD PARAMETERS OF CLARIAS GARIPENUS FED GRADED LEVEL OF MELON SHELL AS REPLACEMENT FOR MAIZE
INTRODUCTION 1.1. Background of the Study Fish is the primary source of protein for about 950 million people worldwide and represents an important part of the…
THE PRODUCTION OF BIODIESEL (FAME) FROM PALM KERNEL OIL (PKO) USING CONCENTRATED SULPHURIC ACID AS CATALYST
CHAPTER ONE INTRODUCTION 1.0 GENERAL INTRODUCTION Energy is a fundamental pillar of modern society as well as being an essential building block for socio-economic development…
EVALUATION OF THE MACRO-NUTRIENT STATUS OF THE CURRENT FIELD PRACTICAL TRAINING (305) EXPERIMENTAL FARMSITE,
ABSTRACT The current 305 Field Practical Training Farm site of the Faculty of Agriculture, University of Benin (Ugbowo), Benin City, Edo state in Nigeria was…
STATISTICAL ANALYSIS ON FERTILITY AND MORTALITY RATE
ABSTACT This project has the aim of studying the fertility and mortality rate in Osogbo Local Government. The study employed the use of statistical methods…
MICROBIOLOGICAL ANALYSIS OF DRY CASSAVA PEEL USED IN THE PREPARATION OF PIG FEEDS
Abstract This study was carried out on the microbiological analysis of dry cassava peel used in the preparation of pigs feed. Since the nutritional compositions…
MULTI-DRUG RESISTANCE PROFILES OF CLINICAL AND ENVIRONMENTAL ISOLATES OF PSEUDOMONAS AERUGINOSA AND ESCHERICHIA COLI
Abstract The emergence of multiple antibiotic resistance in bacteria and the indiscriminate use of antibiotics contribute to the dissemination of resistant pathogens in the environment which may cause problems in therapy and is a serious public health issue. This study was conducted to determine the incidence of Pseudomonas aeruginosa and E.coli isolates in certain clinical and environmental samples as well as to determine the susceptibility patterns of these isolates to some commonly used antibiotics. The organisms were isolated using standard microbiological techniques and the antibiotic susceptibility was determined using disc diffusion method while plasmid curing was done using sodium dodecyl sulphate (SDS). The result of this studies showed that most of the clinical and environmental isolates were more resistant to amoxacillin and augumentin but clinical isolates showed higher resistance. It was also observed that clinical isolates showed least resistance to gentamycin, ofloxacin, and ciprofloxacin; similar least resistance were observed in environmental samples with gentamycin and ciprofloxacin. There was a significant difference (P≥ 0.05) in the percentage resistance between the clinical and environmental isolates. Thirteen isolates that were resistant to more than seven antibiotics were subjected to plasmid curing using 1% and 5% SDS. It was observed that at treatment with 1% SDS some of the isolates became resistant to more than one antibiotic; when SDS was increased to 5%, some of the isolates that were resistant become completely sensitive to all the antibiotics used. However, one of the P.aeruginosa that was initially sensitive to chloramphenicol became completely resistant at 5% SDS and another isolate of P.aeruginosa that was initially sensitive to septrin, sparfloxacin and ciprofloxacin became completely resistant at 1% and 5% SDS. This study indicates that P.aeruginosa and E.coli isolated from clinical samples were more resistant to antibiotics than those isolated from environmental samples. It has as well shown that there may be a possible transfer of resistance from one strain to another. CHAPTER ONE INTRODUCTION AND LITERATURE REVIEW Introduction The discovery of antibacterial agents had a major impact on the rate of survival from infections. However, the changing patterns of antimicrobial resistance caused a demand for new antibacterial agents. Therefore, the emergence of bacterial resistance to most of the commonly used antibiotics is of considerable medical significance (Khan and Malik, 2001; Oteo et al., 2002). Antibiotic resistance genes in most bacteria are frequently found in extra chromosomal elements known as R-plasmids. Pseudomonas aeruginosa is naturally resistant to many of the widely used antibiotics, so chemotherapy is often difficult (Dubois et al., 2001). Resistance is due to a resistance transfer plasmid (R-plasmid) which is a plasmid carrying gene encoding proteins that detoxify various antibiotics (Poole, 2004). Antibiotic resistant bacteria are widespread. Several antibiotic resistant genes can be carried by a single R-plasmid or alternatively, a cell may contain several R plasmids. In either case, the result is multiple resistance (Madigan et al., 2009). Escherichia coli is a Gram negative bacterium and the main aerobic commensal bacterial species (Alhaj et al., 2007; Von and Marre, 2005). The native habitat of Escherichia coli is the enteric tract of humans and other warm-blooded animals. Therefore, Escherichia coli is widely disseminated in the environment through the faeces of humans and other animals and its presence in water is generally considered to indicate faecal contamination and the possible presence of enteric pathogens. Esherichia coli is able to acquire antibiotic resistance easily. Antibiotic resistant Esherichia coli may pass on the genes responsible for antibiotic resistance to other species of bacteria, such as Staphylococcus aureus, through a process called horizontal gene transfer (Dubois et al., 2001). Esherichia coli often carry multidrug resistant plasmids and under stress readily transfer those plasmids to other species. Thus, Esherichia coli is an important reservoir of transferable antibiotic resistance (Salyers et al., 2004). It has been observed that antibiotic susceptibility of bacterial isolates is not constant but dynamic and varies with time and environment (Hassan, 1995)….
ISOLATION AND CHARACTERIZATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT
Abstract This project work was carried on the isolation and characterization of pathogenic bacteria from hawked suya meat. Suya meat were bought from the sellers…
MICROBIAL EXAMINATION OF SPOILT AVOCADO FRUIT
CHAPTER ONE INTRODUCTION 1.1 BACKGROUND OF STUDY The Avocado pear, Persea Americana is a tropical and sub tropical fruit tree, originated in central…
PRODUCTION OF DISINFECTANT
ABSTRACT Disinfectants are antimicrobial agents that are applied to non-living objects to destroy micro-organisms that are living on the object. They are different from other…